Individual Listing

4119C GENOMICS BUILDING
University of California
Riverside, CA 92521

Raikhel, Natasha

Distinguished Professor of Plant Cell Biology
Director of CEPCEB

College of Natural and Agricultural Sciences

Botany & Plant Sciences


Biography

Natasha Raikhel was appointed to the DOE-Plant Research Laboratory at Michigan State University as an Assistant Professor in 1986. There she developed a research program to study the genes involved in nuclear and vacuolar protein sorting in Arabidopsis thaliana. Her promotion to Full Professor was followed by a selection as University Distinguished Professor. She has served on numerous government and industry advisory boards and several editorial boards and was appointed Editor-in-Chief of Plant Physiology starting May 2000. Natasha holds the Ernst and Helen Leibacher Endowed Chair in Plant Molecular, Cell Biology & Genetics, and Distinguished Professor of Plant Cell Biology (CEPCEB)within the UCR Genomics Institue led by Professor Michael Clegg.

Former Institution

Michigan State University-DOE/Plant Research Laboratory and Department of Botany (1986-2001)

Degrees

PhD Cell Biology 1975

Institute of Cytology, Academy of Sciences, Leningrad USSR

M.S. Biology 1970

Leningrad State University, USSR

Awards

1996 Guggenheim Award
2002 Women in Cell Biology Senior Award (American Society for Cell Biology)
2003 Fellow of the American Association for the Advancement of Science (AAAS)
2004 Stephen Hales Prize of ASPB
2007 UCR Faculty Research Lecturer Award
2007 Fellow of ASPB (American Society of Plant Biologists) Award


Research Area

Research in Natasha Raikhel’s laboratory is focused on understanding the fundamental principles of vacuolar biogenesis (Rojo et al., 2001) and protein trafficking through the secretory system (Sanderfoot et al., 2001) and on elucidation of the components that mediate cell wall biosynthesis in plants (Faik et al., 2002).

Publications

Selected Publications Related to Vacuolar Trafficking

Chary SN, Hicks GR, Choi YG, Carter D, and Raikhel NV (2008)
Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis. Plant Physiol 146: 97-107.
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Sohn EJ, Rojas-Pierce M, Pan S, Carter C, Serrano-Mislata A, Madueno F, Rojo E, Surpin M, Raikhel NV (2007) The shoot meristem identity gene TFL1 is involved in flower development and trafficking to the protein storage vacuole. Proc Natl Acad Sci USA 104: 18801-18806.

Rojas-Pierce M, Titapiwatanakun B, Sohn EJ, Fang F, Larive CK, Blakeslee J, Cheng Y, Cutler S, Peer WA, Murphy AS and Raikhel NV (2007) Arabidopsis P-Glycoprotein19 Participates in the Inhibition of Gravitropism by Gravacin. Chem & Biol 14: 1366-1376.
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Zou Y, Rojas-Pierce M, Raikhel NV and Pirrung M (2007). Preparation of Methyl Ester Precursors of Biologically Active Agents. Biotechniques (in press).

Norambuena L, Zouhar J, Hicks GR and Raikhel NV (2007) Identification of cellular pathways affected by Sortin2, a synthetic compound that affects protein targeting to the vacuole in Saccharomyces cerevisiae BMC Chemical Biology (in press).

Robert S, Zouhar J, Carter CJ and Raikhel NV (2007) Isolation of intact vacuoles from Arabidopsis rosette leaf-derived protoplasts. Nature Protocols 2: 259-262.
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Sanmartín M, Ordóñez A, Sohn EJ, Robert S, Sánchez-Serrano JJ, Surpin MA, Raikhel M+NV, and Rojo E (2007) Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis. PNAS 104: 3645-3650.
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Raikhel N and Hicks G (2007) Signaling from Plant Endosomes: Compartments with Something to Say! Genes & Dev 21:1578-1580.
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Pan S*, Carter CJ*, and Raikhel NV (2005) Understanding Protein Trafficking in Plant Cells through Proteomics (* equal contribution). Expert Rev Proteomics 2(5):781-792.
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Girke T, Cheng LC and Raikhel NV (2005) ChemMine: A Compound Mining Database for Chemical Genomics. Plant Physiol 138:573-577.
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Horan K, Lauricha J, Bailey-Serres J, Raikhel NV, Girke T. (2005) Genome Cluster Database. A Sequence Family Analysis Platform for Arabidopsis and Rice. Plant Physiol 138: 47-54.
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Surpin M, Rojas-Pierce M, Carter C, Hicks GR, Vasquez, Raikhel NV (2005) The Power of Chemical Genomics to Study the Link between Endomembrane System Components and the Gravitropic Response. Proc Natl Acad Sci USA 102:4902-4907.
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Carter C, Pan S, Zouhar J, Avila EL, Girke T, and Raikhel NV (2004) The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins. Plant Cell 16:3285-3303.
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Rojo E, Martin R, Carter C, Zouhar J, Pan S, Plotnikova J, Jin H, Paneque M, Sanchez-Serrano JJ, Baker B, Ausubel FM, Raikhel NV (2004) Warrior and Executioner: VPEγ Exhibits a Caspase-like Activity that Contributes to Defense Against Pathogens. Curr Biol 14: 1897-1906.
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Carter CJ, Bednarek SY, Raikhel NV (2004) Membrane trafficking in plants: new discoveries and new approaches. Curr Opin Plant Biol 7:701-707.
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Zouhar J, Hicks G, Raikhel NV (2004) Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis. Proc Natl Acad Sci USA 101:9497-9501.
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Hicks GR, Rojo E, Hong S, Carter D, Raikhel NV (2004) Geminating Pollen Has Tubular Vacuoles, Displays Highly Dynamic Vacuole Biogenesis, and Requires VACUOLESS1 for Proper Function. Plant Physiol 134: 1227-1239.
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Surpin M, Raikhel NV (2004) Traffic Jams Affect Plant Development and Signal Transduction. Nature Reviews Mol Cell Biol 5: 100-109.
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Avila EL, Zouhar J, Agee AE, Carter DG, Chary SN, Raikhel NV (2003) Tools to Study Plant Organelle Biogenesis. Point Mutation Lines with Disrupted Vacuoles and High-Speed Confocal Screening of Green Fluorescent Protein-Tagged Organelles. Plant Physiol 133: 1673-1676.
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Surpin M, Zheng Haiyan, Morita MT, Saito C, Avila E, Blakeslee JJ, Bandyopadhyay A, Kovaleva V, Carter D, Murphy A, Tasaka M, Raikhel NV (2003) The VTI Family of SNARE Proteins Is Necessary for Plant Viability and Mediates Different Protein Transport Pathways. Plant Cell 15: 2885-2899.
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Rojo E, Zouhar J, Carter C, Kovaleva V, Raikhel NV (2003) A Unique Mechanism for Protein Processing and Degradation in Arabidopsis Thaliana. Proc. Natl. Acad. Sci. USA 100: 7389-7394.
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Rojo E, Zouhar J, Kovaleva V, Hong S, Raikhel NV (2003) The AtC-VPS Protein Complex is Localized to the Tonoplast and the Prevacuolar Compartment in Arabidopsis. Mol Biol Cell 14: 361-369.
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Sanderfoot AA, Raikhel NV (2002) The Secretory System of Arabidopsis, The Arabidopsis Book, eds. Somerville CR and Meyerowitz EM, American Society of Plant Biologists, Rockville, MD. DOI 10.1199/tab.0098.
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Price NJ, Rieter, W-D, Raikhel NV (2002) Glycosyltransferases Involved in Polysaccharide Biosynthesis, The Arabidopsis Book, eds. Somerville CR and Meyerowitz EM, American Society of Plant Biologists, Rockville, MD, DOI 10.1199/tab.0025.
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Zheng H, Bednarek SY, Sanderfoot AA, Alonso J, Ecker JR, Raikhel NV (2002) NPSN11 is a Cell Plate-Associated SNARE Protein That Interacts with the Syntaxin KNOLLE. Plant Physiol 129: 530-539.
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Rojo E, Sharma VK, Kovaleva V, Raikhel NV, Fletcher JC (2002) CLV3 is Localized to the Extracellular Space, Where It Activates the Arabidopsis CLAVATA Stem Cell Signaling Pathway. Plant Cell 14: 969-977.
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Sanderfoot, AA, Pilgrim M, Adam L, Raikhel NV (2001) Disruption of individual members of Arabidopsis syntaxin gene families indicates each have essential functions. Plant Cell 13: 659-666.
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Sanderfoot AA, Kovaleva V, Bassham DC, Raikhel NV (2001) Interactions between syntaxins identify at least five SNARE complexes within the Golgi/Prevacuolar system of the Arabidopsis cell. Mol Biol Cell 12:3733-3743.
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Rojo E, Gillmor CS, Kovaleva V, Somerville CR, Raikhel NV (2001) VACUOLELESS1 is an essential gene required for vacuole formation and morphogenesis in Arabidopsis. Dev Cell 1: 303-310.
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Sanderfoot AA, Assaad FF, Raikhel NV (2000) The Arabidopsis Genome. An abundance of Soluble N-Ethylmaleimide-Sensitive factor adaptor protein receptors. Plant Physiol 124: 1558-1569.
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Bassham DC, Sanderfoot AA, Kovaleva V, Zheng HY, Raikhel NV (2000) AtVPS45 complex formation at the trans-Golgi network. Mol Biol Cell 11, 2251-2265.
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Bassham DC, Raikhel NV (2000) Unique features of the plant vacuolar sorting machinery. Curr Opin Cell Biol 12: 491-495.

Bassham DC, Raikhel NV (2000) Plant cells are not just green yeast. Plant Physiol 122: 999-1001.
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Ahmed SU, Rojo E, Kovaleva V, Venkataraman S, Dombrowski JE, Matsuoka K, Raikhel NV (2000) The plant vacuolar sorting receptor AtELP is involved in transport of NH2-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana. J Cell Biol 149: 1335-1344.
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Zheng H, Fischer von Mollard G, Kovaleva V, Stevens TH, Raikhel NV (1999) The plant vesicle-associated SNARE AtVTI1a likely mediates vesicle transport from the trans-Golgi network to the prevacuolar compartment. Mol Biol Cell 10: 2251-2264
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Vitale A, Raikhel NV (1999) What do proteins need to reach different vacuoles? Trends Plant Sci 4: 149-155.





Selected Publications Related to Cell Wall Metabolism

Perrin RM, DeRocher AE, Bar-Peled M, Zeng W, Norambuena L, Orellana A, Raikhel NV, Keegstra K (1999) Xyloglucan Fucosyltransferase, an Enzyme Involved in Plant Cell Wall Biosynthesis. Science 284: 1976-1979.

Faik A, Bar-Peled M, DeRocher AE, Zeng WQ, Perrin RM, Wilkerson C, Raikhel NV, Keegstra K (2000) Biochemical Characterization and Molecular Cloning of an -x-1,2-Fucosyltransferase
that Catalyzes the Last Step of Cell Wall Xyloglucan Biosynthesis in Pea. J. Biol. Chem. 275: 15082-15089.

Keegstra K and Raikhel NV (2001) Plant glycosyltransferases Curr. Opin Plant Biol 4: 219-224.

Sarria R, Wagner TA, Neill MO, Faik A, Wilkerson C, Keegstra K, and Raikhel NV(2001) Characterization of a Family of Arabidopsis Genes Related to Xyloglucan Fucosyltransferase1. Plant Physiol 127: 1595-1606.

Faik A, Price NF, Raikhel NV, Keegstra K (2002) Arabidopsis Gene Encoding an x-Xylosyltrasferase Involved in Xyloglucan Biosynthesis. Proc Natl Acad Sci USA. 99: 7797-7802

Vanzin GF, Madson M, Carpita NC, Raikhel, NV, Keegstra K, and Reiter WD (2002) The mur2 mutant of Arabidopsis thaliana lacks fucoslyated xyloglucan because of a lesion in fucosyltransferase AtFUT1. Proc. Natl. Acad. Sci. USA 99:3340-3345.

Selected Publications Related to Cell Wall Metabolism

Cocuron J-C, Lerouxel O, Drakakaki G, Alonso AP, Liepman AH, Keegstra K, Raikhel NV, and Wilkerson CG (2007) A gene from the cellulose synthase-like C family encodes a ß-1,4 glucan synthase. Proc Natl Acad Sci USA104:8550-8555.
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Girke T, Lauricha J, Tran H, Keegstra K, Raikhel NV (2004) The Cell Wall Navigator Database: A Systems-Based Approach to Organism-Unrestricted Mining of Protein Families Involved in Cell Wall Metabolism. Plant Physiol 136:3003-3008.
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Vanzin GF, Madson M, Carpita NC, Raikhel, NV, Keegstra K, and Reiter WD (2002) The mur2 mutant of Arabidopsis thaliana lacks fucoslyated xyloglucan because of a lesion in fucosyltransferase AtFUT1. Proc. Natl. Acad. Sci. USA 99:3340-3345.
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Faik A, Price NF, Raikhel NV, Keegstra K (2002) Arabidopsis Gene Encoding an α-xylosyltransferase Involved in Xyloglucan Biosynthesis. Proc Natl Acad Sci USA 99: 7797-7802.
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Sarria R, Wagner TA, Neill MO, Faik A, Wilkerson C, Keegstra K, and Raikhel NV(2001) Characterization of a family of Arabidopsis genes related to xyloglucan fucosyltransferase. Plant Physiol 127: 1595-1606.
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Keegstra K and Raikhel NV (2001) Plant glycosyltransferases Curr Opin Plant Biol 4: 219-224.
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Faik A, Bar-Peled M, DeRocher AE, Zeng WQ, Perrin RM, Wilkerson C, Raikhel NV, Keegstra K (2000) Biochemical characterization and molecular cloning of an -(1,2) fucosyltransferase that catalyzes the last step of cell wall xyloglucan biosynthesis in pea. J Biol Chem 275: 15082-15089.
Full Text PDF

Perrin RM, DeRocher AE, Bar-Peled M, Zeng W, Norambuena L, Orellana A, Raikhel NV, Keegstra K (1999) Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis. Science 284: 1976-1979.
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Invited Contributions and Book Chapters

Norambuena, L., Hicks, G. and Raikhel N.V. (2007) The use of chemical genomics to investigate pathways intersecting auxin-dependent responses and endomembrane trafficking in Arabidopsis thaliana. In, Methods in Molecular Biology series. Dario Bonetta, ed. Humana Press. (in press)

Price NJ, Reiter WD, Raikhel NV (2002) Glycosyltransferases Involved in Polysaccharide Biosynthesis, The Arabidopsis Book, eds. C.R. Somerville and E.M. Meyerowitz, American Society of Plant Biologists, Rockville, MD, DOI 10.1199/tab.0025.

Sanderfoot AA, Raikhel NV (2002) The Secretory System of Arabidopsis, The Arabidopsis Book, eds. C.R. Somerville and E.M. Meyerowitz, American Society of Plant Biologists, Rockville, MD, DOI 10.1199/tab.0098.

Cech TR, Eddy SR, Eisenberg D, Hersey K, Holtzman SH, Poste GH, Raikhel NV, Scheller RH, Singer DB, Waltham MC (2002) The National Academy of Sciences-Sponsored Report: "Sharing Publication-Related Data and Materials: Responsibilities of Authorship in the Life Sciences." ed. Grossblatt N, The National Academies Press, Washington DC.