Individual Listing

BOYCE HALL
University of California
Riverside, CA 92521

Henry, Helen L

Professor of Biochemistry & Biochemist, Emeritus

College of Natural and Agricultural Sciences

Biochemistry


Biography

Research Specialization - My research is concerned with the regulation of steroid hormones at the point of synthesis. Specifically, we are examining, at the cellular and molecular level, the regulation of the production of the calcium regulating steroid hormone, 1,25dihydroxyvitamin D3, (1,25(OH)2D3) whose synthesis is catalyzed by a mitochondrial cytochrome P450-dependent mixed function oxidase. Our studies include activation of both protein kinases A and C, processes already implicated in the regulation of this enzyme. Another major area of interest is the molecular structure and regulation of the gene encoding the endogenous inhibitor protein of cyclic AMP-dependent protein kinase (PKI). We have determined that the steady state levels PKI mRNA is regulated by 1,25(OH)2D3 in vivo and in cell culture. We have obtained the genomic DNA which encodes PKI, determined its intron/exon structure, and are currently studying the regulatory elements in its promoter region. These studies are directed not only at unraveling the interaction of calcium regulating hormones at the cellular and molecular levels, but also are aimed toward the development of a model system for the examination of the regulation of steroid metabolism in general in a tissue other than those of well known steroidogenic capacity such as the adrenal cortex.

Degrees

PhD Biology 1970

Washington University

BA Biology 1965

Washington University

Awards

2003 American Association for the Advancement of Science, Biological Sciences
1984 American Society for Bone and Mineral Research Fuller Albright Award
1978-1983 Public Health Service Career Development Award
NIH General Medicine B Study Section
Editorial Boards of Endocrinology and American Journal of Physiology
Council, American Society Bone & Mineral Research


Research Area

Molecular Endocrinology

Publications

Rowland-Goldsmith, M. A., Holmquist, B., and Henry, H.L. Genomic cloning, structure, and regulatory elements of the 1a-25(OH)2D3 down-regulated gene for the cyclic AMP-dependent protein kinase inhibitor. Biochim. Biophys., Acta (in press, 1999)

Henry, H.L. The 25-Hydroxyvitamin D3-1a-Hydroxylase in Vitamin D (ed.D., Feldman, F. H. Glorieux, J. W. Pike) P57-68, Academic Press, 1997

Marchetto, G.S. and Henry, H.L. Tissue-specific expression and regulation by 1,25(OH)2D3 of chick kidney protein kinase inhibitor mRNA. Endocrine 6:5-10 (1997).

Xu, J. and Henry, H.L. Tissue-specific regulation by vitamin D3 of a novel protein containing ankyrin-like repeats. Mol. Cell Endocr. 126:101-107 (1997).

Blanchard, R.K. and Henry, H. L. Chick kidney ferredoxin: Complementary DNA cloning and vitamin D effects on mRNA levels. Comp. Biochem. Physiol. 114:337-344 (1996).

Chou, S.Y., Hannah, S.S., Lowe, K.E., Norman, A.W. and Henry, H.L. Tissue-specific regulation by vitamin D status of nuclear and mitochondrial gene expression in kidney and intestine. Endocrinology 136:5520-5526 (1995).

Marchetto, G.M. and Henry, H.L. Cloning and sequencing of the cDNA encoding the avian kidney cAMPdependent protein kinase inhibitor. Gene 158:303-304 (1995).

Tang, C. and Henry, H.L. Activation of protein kinase C stimulates dephosphorylation of chick renal mitochondrial ferredoxin. Endocrinology 133:1823-1829 (1993).

Tang, C. and Henry, H.L. Over-expression in Escherichia coli and affinity purification of check kidney ferredoxin. J. Biol. Chem. 268:5069-5076 (1993).

Henry, H.L. Vitamin D hydroxylases. J. Cell. Biochem. 49:4-9 (1992).