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Henry, Helen L
Professor of Biochemistry & Biochemist, Emeritus
Research Specialization - My research is concerned with the regulation of steroid hormones at the point of synthesis. Specifically, we are examining, at the cellular and molecular level, the regulation of the production of the calcium regulating steroid hormone, 1,25dihydroxyvitamin D3, (1,25(OH)2D3) whose synthesis is catalyzed by a mitochondrial cytochrome P450-dependent mixed function oxidase. Our studies include activation of both protein kinases A and C, processes already implicated in the regulation of this enzyme. Another major area of interest is the molecular structure and regulation of the gene encoding the endogenous inhibitor protein of cyclic AMP-dependent protein kinase (PKI). We have determined that the steady state levels PKI mRNA is regulated by 1,25(OH)2D3 in vivo and in cell culture. We have obtained the genomic DNA which encodes PKI, determined its intron/exon structure, and are currently studying the regulatory elements in its promoter region. These studies are directed not only at unraveling the interaction of calcium regulating hormones at the cellular and molecular levels, but also are aimed toward the development of a model system for the examination of the regulation of steroid metabolism in general in a tissue other than those of well known steroidogenic capacity such as the adrenal cortex.
2003 American Association for the Advancement of Science, Biological Sciences
Rowland-Goldsmith, M. A., Holmquist, B., and Henry, H.L. Genomic cloning, structure, and regulatory elements of the 1a-25(OH)2D3 down-regulated gene for the cyclic AMP-dependent protein kinase inhibitor. Biochim. Biophys., Acta (in press, 1999)