Personal Web Site
2320 SPIETH HALL
University of California
Riverside, CA 92521
(951) 827-3768 (Voice)
(951) 827-4286 (Fax)
(951) 827-5903 (Dept)
Professor of Cell Biology
Director, UCR Stem Cell Center and Stem Cell Core Facility
- College of Natural and Agricultural Sciences
- Cell Biology & Neuroscience
- M.A. Biology 1968
- Wellesley College
- Ph.D. Biology 1972
- University of Houston
Elected a Fellow of the AAAS, 2001
Recipient of Chancellor's Award for Excellence in Mentoring Undergraduate Research, 1998-99
My laboratory studies reproductive biology and developmental toxicology using mammalian systems. Our long term goal is to use human embryonic stem cells to prevent disease. Much of our current work is done using mouse and human embryonic stem cells to model the pre and post-implantation stages of development. We are developing new assays that can detect and identify environmental toxicants and drugs before they can cause harm to humans. Because human embryonic stem cells are difficult to use in toxicological assays, we are developing a new platforms using state-of-the-art equipment to study morphological and molecular endpoints in video data collected from stem cells during various experimental conditions. The video data are a rich source of information that is being mined using video bioinformatics technology. This involves the development and use of computer software to extract endpoint information from videos. We are currently working in conjunction with faculty in engineering to apply video bioinformatics principles to our stem cells data.
We recently used mouse embryonic stem cells to evaluate the toxicity of smoke from traditional and harm reduction cigarettes. Harm reduction brands are generally considered and sometimes advertized as safer than the traditional cigarettes. The mouse embryonic stem cell assays revealed that smoke from the harm reduction brands was at least as toxic and often more toxic than traditional smoke. We are currently extending these studies to human embryonic stem cells.
We also study reproductive toxicology using the hamster oviduct as a model to evaluate harm to the female reproductive tract. Our lab has developed an explant assay the gives four endpoints related to oviductal functioning. This assay has been used by our lab in the past to evaluate the effects of cigarette smoke on reproductive function. We have found that sidestream smoke which burns off the ends of cigarettes is more harmful in the oviductal bioassays then mainstream smoke that active smokers inhale.
Our lab also works on differentiation of oocytes from stem cells. The ability to differentiate oocytes in vitro provides an excellent model for understanding the complex process of mammalian oocyte development and could eventually provide a source of oocytes for infertile couples.
Talbot, P. Sabrina Lin. Cigarette Smoke’s Effect on Fertilization and Pre-implantation Development: Assessment Using Animal Models, Clinical Data, and Stem Cells.
Journal of Biological Sciences (in press).
Tartia, A.P., N. Rudraraju, T,Richards, M. Hammer, P. Talbot, and J. Baltz. Cell volume regulations is initiated in mouse oocytes after ovulation. Development 136:2247-2254 (2009)
S. Lin, V. Tran, P. Talbot. Comparison of toxicity of smoke from traditional and harm-reduction cigarettes using mouse embryonic stem cells as a novel model for pre-implantation development. Human Reproduction, 24:386-397 (2009)
P. Talbot. In Vitro Assessment of Reproductive Toxicity of Tobacco Smoke and Its Constituents. Birth Defects Research (Part C) 84:61-72 (2008)
Baibakov, Lyn Gauthier,P. Talbot, Tracy L. Rankin and Jurrien Dean. Sperm binding to the zona pellucida is not sufficient to induce acrosome exocytosis. Development 134, 933-943 (2007) doi:10.1242/dev.02752
P. Talbot, L. Fernandez. Scrambling the Egg Origin Dogma.Regenerative Medicine 2006; Volume 1, Number 5, September 2006, pp. 693-695(3)
K. Riveles, V. Tran, Ryan Roza, Derek Kwan, P. Talbot. Smoke from Traditional Commercial, Harm Reduction, and Research Brand Cigarettes Impairs Oviductal Functioning in Hamsters (Merocricetus auratus) In Vitro. Human Reproduction 2006; doi: 10.1093/humrep/del380
Richard Yu, M. Wu, S.Lin, Prue Talbot. Cigarette Smoke Toxicants Alter Growth and Survival of Cultured Mammalian Cells. Toxicological Sciences 2006; 93(1): 82-95.
Talbot P, Riveles K. Smoking and Reproduction: The Oviduct as a Target of Cigarette Smoke. Reproductive Biology and Endocrinology 2005; 3:25.
Liu M, Oh A, Calarco P, Yamada M, Coonrod SA, and Talbot P. Peptidylarginine Deiminase (PAD) is a Mouse Cortical Granule Protein that Plays a Role in Preimplantation Embryonic Development. Reproductive Biology and Endocrinology 2005; 3:24.
Gieseke C, and Talbot P. Cigarette Smoke Inhibits Hamster Oocyte Pickup by Increasing Adhesion between the Oocyte Cumulus Complex and Oviductal Cilia.
Biology of Reproduction 2005; 73(3): 443-451.